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Metabolic flux analysis (MFA) is an experi … Metabolic flux analysis (MFA) is an experimental fluxomics technique used to examine production and consumption rates of metabolites in a biological system. At an intracellular level, it allows for the quantification of metabolic fluxes, thereby elucidating the central metabolism of the cell. Various methods of MFA, including isotopically stationary metabolic flux analysis, isotopically non-stationary metabolic flux analysis, and thermodynamics-based metabolic flux analysis, can be coupled with stoichiometric models of metabolism and mass spectrometry methods with isotopic mass resolution to elucidate the transfer of moieties containing isotopic tracers from one metabolite into another and derive information about the metabolic network. Metabolic flux analysis (MFA) has many applications such as determining the limits on the ability of a biological system to produce a biochemical such as ethanol, predicting the response to gene knockout, and guiding the identification of bottleneck enzymes in metabolic networks for metabolic engineering efforts. Metabolic flux analysis may use 13C-labeled isotope tracers for isotopic labeling experiments. Nuclear magnetic resonance (NMR) techniques and mass spectrometry may then be used to measure metabolite labeling patterns to provide information for determination of pathway fluxes. Because MFA typically requires rigorous flux calculation of complex metabolic networks, publicly available software tools have been developed to automate MFA and reduce its computational burden.e MFA and reduce its computational burden.
, Análise de fluxo metabólico (AFM) é uma té … Análise de fluxo metabólico (AFM) é uma técnica experimental de usada para examinar as taxas de produção e consumo de metabólitos em um sistema biológico. No nível intracelular, permite a quantificação dos metabólitos, elucidando o metabolismo central da célula. Empregando de métodos de metabolismo e espectrometria de massa com resolução isotópica de massa, a transferência de contendo marcadores isotópicos de um metabólito para outro pode ser elucidada, e informações sobre a rede metabólica são derivadas. A análise de fluxo metabólico tem muitas tem muitas aplicações, como determinar os limites da capacidade de um sistema biológico em produzir um composto bioquímico como o etanol e prever a resposta a adições ou nocaute de genes (inativação). adições ou nocaute de genes (inativação).
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Metabolic flux analysis (MFA) is an experi … Metabolic flux analysis (MFA) is an experimental fluxomics technique used to examine production and consumption rates of metabolites in a biological system. At an intracellular level, it allows for the quantification of metabolic fluxes, thereby elucidating the central metabolism of the cell. Various methods of MFA, including isotopically stationary metabolic flux analysis, isotopically non-stationary metabolic flux analysis, and thermodynamics-based metabolic flux analysis, can be coupled with stoichiometric models of metabolism and mass spectrometry methods with isotopic mass resolution to elucidate the transfer of moieties containing isotopic tracers from one metabolite into another and derive information about the metabolic network. Metabolic flux analysis (MFA) has many applications slux analysis (MFA) has many applications s
, Análise de fluxo metabólico (AFM) é uma té … Análise de fluxo metabólico (AFM) é uma técnica experimental de usada para examinar as taxas de produção e consumo de metabólitos em um sistema biológico. No nível intracelular, permite a quantificação dos metabólitos, elucidando o metabolismo central da célula. Empregando de métodos de metabolismo e espectrometria de massa com resolução isotópica de massa, a transferência de contendo marcadores isotópicos de um metabólito para outro pode ser elucidada, e informações sobre a rede metabólica são derivadas. A análise de fluxo metabólico tem muitas tem muitas aplicações, como determinar os limites da capacidade de um sistema biológico em produzir um composto bioquímico como o etanol e prever a resposta a adições ou nocaute de genes (inativação). adições ou nocaute de genes (inativação).
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Análise de fluxo metabólico
, Metabolic flux analysis
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