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A parallel-plate fluid flow chamber is a b … A parallel-plate fluid flow chamber is a benchtop (in vitro) model that simulates fluid shear stresses on various cell types exposed to dynamic fluid flow in their natural, physiological environment. The metabolic response of cells in vitro is associated with the wall shear stress. A typical parallel-plate flow chamber consists of a polycarbonate distributor, a silicon gasket, and a glass coverslip. The distributor, forming one side of the parallel-plate flow chamber, includes inlet port, outlet port, and a vacuum slot. The thickness of the gasket determines the height of the flow path. The glass coverslip forms another side of the parallel-plate flow chamber and can be coated with extracellular matrix (ECM) proteins, vascular cells, or biomaterials of interest. A vacuum forms a seal to hold these three parts and ensures a uniform channel height. Typically, the fluid enters one side of the chamber and leaves from an opposite side. The upper plate is usually transparent while the bottom is a prepared surface on which the cells have been cultured for a predetermined period. Cell behavior is viewed with either transmitted or reflective light microscope.ransmitted or reflective light microscope.
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A parallel-plate fluid flow chamber is a b … A parallel-plate fluid flow chamber is a benchtop (in vitro) model that simulates fluid shear stresses on various cell types exposed to dynamic fluid flow in their natural, physiological environment. The metabolic response of cells in vitro is associated with the wall shear stress. Typically, the fluid enters one side of the chamber and leaves from an opposite side. The upper plate is usually transparent while the bottom is a prepared surface on which the cells have been cultured for a predetermined period. Cell behavior is viewed with either transmitted or reflective light microscope.ransmitted or reflective light microscope.
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