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Técnicas biofísicas são métodos usados par … Técnicas biofísicas são métodos usados para obter-se informação sobre sistemas biológicos no um nível atômico ou molecular. Elas sobrepõe-se com métodos de outros campos da ciência. As principais técnicas biofísicas são:
* Dicroísmo circular, um método de detecçao de grupos quirais em moléculas, especialmente para determinar a .
* Química computacional, o uso de métodos numéricos para obter-se a estrutura e equilíbrio dinâmico em sistemas biológicos.
* , uma técnica analítica usada para medir-se a conformação e atividade em tempo real de uma larga variedade de biomoléculas e suas interações.
* Microscópio eletrônico, para obter imagens de alta resolução de estruturas subcelulares
* Espectroscopia de fluorescência, a qual pode ser usada para detectar rearranjos estruturais, assim como interações de biomoléculas.
* analisa as propriedades mecânicas de moléculas individuais ou arranjos macromoleculares usando pequenos cantilevers flexíveis, focados com luz laser, ou campos magnéticos.
* Eletroforese em gel, aqual é usada para determinar a massa, a carga e as interações de moléculas biológicas.
* Calorimetria de titulação isotérmica (ITC, do inglês Isothermal Titration Calorimetry) a qual mede os efeitos do calor relacionado às interações.
* Espectrometria de massa é uma técnica que fornece a massa molecular com grande precisão.
* Microscopia, por exemplo usando instrumentos com laser para escaneamento e transmissão.
* Pinça óptica e permite a manipulaçao de moléculas isoladas, fornecendo informações sobre ADN (DNA) e sua interação com proteínas e motores moleculares, tais como helicase e ARN-polimerase.
* Ressonância magnética nuclear de proteínas, fornecendo informação sobre a estrutura exata de moléculas biológicas, assim como sua dinâmica.
* (single molecule spectroscopy) é um termo geral aplicado à uma classe de técnicas que são sensíveis o suficiente para detectar moléculas isoladas e frequentemente incorpora detecção fluorescente.
* (SAXS, small angle X-ray scattering) é uma técnica que fornece uma aproximação de baixa resolução da estrutura molecular.
* Espectrofotometria, a medição da transmissão de luz através de diferentes soluções ou substâncias a diferentes comprimentos de onda de luz. A colorimetria é um exemplo disto.
* Ultracentrifugação, a qual fornece informação sobre a forma e massa de moléculas
* várias técnicas de cromatografia, as quais são usadas para a purificação e análise de moléculas biológicas.
* Cristalografia de raios X, outro método para obter-se acesso à estrutura exata de moléculas com resolução atômica. Ver também: biofísicam resolução atômica. Ver também: biofísica
, Біофізичні методи — набір методів, які використовуються для дослідження фізичних властивостей біологічних систем.
, The following outline is provided as an overview of and topical guide to biophysics: Biophysics – interdisciplinary science that uses the methods of physics to study biological systems.
, إن المخطط التالي عبارة عن نظرة شاملة على مواضيع الفيزياء الحيوية:فيزياء حيوية— هي تداخل التخصصات و تستخدم الطرق الفيزيائية لدارسة النظام الأحيائي
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| rdfs:comment |
Біофізичні методи — набір методів, які використовуються для дослідження фізичних властивостей біологічних систем.
, Técnicas biofísicas são métodos usados para obter-se informação sobre sistemas biológicos no um nível atômico ou molecular. Elas sobrepõe-se com métodos de outros campos da ciência. As principais técnicas biofísicas são: Ver também: biofísica
, إن المخطط التالي عبارة عن نظرة شاملة على مواضيع الفيزياء الحيوية:فيزياء حيوية— هي تداخل التخصصات و تستخدم الطرق الفيزيائية لدارسة النظام الأحيائي
, The following outline is provided as an overview of and topical guide to biophysics: Biophysics – interdisciplinary science that uses the methods of physics to study biological systems.
|
| rdfs:label |
Біофізичні методи
, Técnicas biofísicas
, Outline of biophysics
, مخطط الفيزياء الحيوية
|
