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HgcC is a small non coding RNA (ncRNA). It … HgcC is a small non coding RNA (ncRNA). It is the functional product of a gene which is not translated into protein. This ncRNA gene was originally identified by computationally searching the genome of the thermophilic archea Methanococcus jannaschii for non-coding regions of high guanine-cytosine (GC) content. The original rational for this search was based on the observation that the genomes of these bacteria are adenosine-thiamine (AT) rich and consequently have a low GC content. However, the GC content of ribosomal RNA (rRNA) and transfer RNA (tRNA) genes in hyperthermophiles shows a strong correlation with optimal growth temperature. It was proposed that non coding regions of high GC-content might encode functional RNA products. The computational screen identified a number of novel ncRNA genes in the genome of M.jannaschii. These were named hgc- ("high GC") A, B, C, D, E, F and G. Two other homologues were detected called HhcA and HhcB after "homologue of hgcC". A further RNA element, SscA RNA, was also identified. The HgcC gene product was experimentally validated by Northern blot and RACE-PCR analysis. The function of this ncRNA is unknown.is. The function of this ncRNA is unknown.
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Predicted secondary structure and sequence conservation of HgcC
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HgcC family RNA
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HgcC
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rdfs:comment |
HgcC is a small non coding RNA (ncRNA). It … HgcC is a small non coding RNA (ncRNA). It is the functional product of a gene which is not translated into protein. This ncRNA gene was originally identified by computationally searching the genome of the thermophilic archea Methanococcus jannaschii for non-coding regions of high guanine-cytosine (GC) content. The original rational for this search was based on the observation that the genomes of these bacteria are adenosine-thiamine (AT) rich and consequently have a low GC content. However, the GC content of ribosomal RNA (rRNA) and transfer RNA (tRNA) genes in hyperthermophiles shows a strong correlation with optimal growth temperature. It was proposed that non coding regions of high GC-content might encode functional RNA products. The computational screen identified a number of novel ncnal screen identified a number of novel nc
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rdfs:label |
HgcC family RNA
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