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Affinity electrophoresis is a general name … Affinity electrophoresis is a general name for many analytical methods used in biochemistry and biotechnology. Both qualitative and quantitative information may be obtained through affinity electrophoresis. The methods include the so-called electrophoretic mobility shift assay, charge shift electrophoresis and affinity capillary electrophoresis. The methods are based on changes in the electrophoretic pattern of molecules (mainly macromolecules) through biospecific interaction or complex formation. The interaction or binding of a molecule, charged or uncharged, will normally change the electrophoretic properties of a molecule. Membrane proteins may be identified by a shift in mobility induced by a charged detergent. Nucleic acids or nucleic acid fragments may be characterized by their affinity to other molecules. The methods have been used for estimation of binding constants, as for instance in lectin affinity electrophoresis or characterization of molecules with specific features like glycan content or ligand binding. For enzymes and other ligand-binding proteins, one-dimensional electrophoresis similar to counter electrophoresis or to "rocket immunoelectrophoresis", affinity electrophoresis may be used as an alternative quantification of the protein. Some of the methods are similar to affinity chromatography by use of immobilized ligands.omatography by use of immobilized ligands.
, Die Affinitätselektrophorese umfasst in de … Die Affinitätselektrophorese umfasst in der Biochemie alle elektrophoretischen Verfahren, bei denen ein verändertes Laufverhalten von Molekülen (z. B. von Proteinkomplexen oder DNA-Protein-Komplexen) durch die Affinität zweier oder mehrerer Moleküle zueinander erzeugt wird. Aufgrund der veränderten Laufgeschwindigkeiten werden affinitätselektrophoretische Verfahren auch als electrophoretic shift assays bezeichnet.s electrophoretic shift assays bezeichnet.
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Affinity electrophoresis is a general name … Affinity electrophoresis is a general name for many analytical methods used in biochemistry and biotechnology. Both qualitative and quantitative information may be obtained through affinity electrophoresis. The methods include the so-called electrophoretic mobility shift assay, charge shift electrophoresis and affinity capillary electrophoresis. The methods are based on changes in the electrophoretic pattern of molecules (mainly macromolecules) through biospecific interaction or complex formation. The interaction or binding of a molecule, charged or uncharged, will normally change the electrophoretic properties of a molecule. Membrane proteins may be identified by a shift in mobility induced by a charged detergent. Nucleic acids or nucleic acid fragments may be characterized by their affingments may be characterized by their affin
, Die Affinitätselektrophorese umfasst in de … Die Affinitätselektrophorese umfasst in der Biochemie alle elektrophoretischen Verfahren, bei denen ein verändertes Laufverhalten von Molekülen (z. B. von Proteinkomplexen oder DNA-Protein-Komplexen) durch die Affinität zweier oder mehrerer Moleküle zueinander erzeugt wird. Aufgrund der veränderten Laufgeschwindigkeiten werden affinitätselektrophoretische Verfahren auch als electrophoretic shift assays bezeichnet.s electrophoretic shift assays bezeichnet.
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Affinity electrophoresis
, Affinitätselektrophorese
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